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criterion xt tris acetate gels  (Bio-Rad)


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    Structured Review

    Bio-Rad criterion xt tris acetate gels
    Criterion Xt Tris Acetate Gels, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 21519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/criterion+xt+tris+acetate+gels/Tris/pm41986367-470-42-46
    Average 98 stars, based on 21519 article reviews
    criterion xt tris acetate gels - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    SDS Page:

    Article Title: MCM Double Hexamer Loading Visualised with Human Proteins
    Article Snippet: A sodium chloride wash was performed by washing 2x for 2 min, 1,250 rpm with bead-assay buffer + sodium chloride as indicated, followed by a LS wash. For elution, DNA was degraded by nuclease treatment by resuspending the beads in bead-assay buffer + 2 mM calcium chloride, 600 u micrococcal nuclease (NEB, M0247S) and incubation at 37°C for 2 min with 1,250 rpm shaking. .. The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad), followed by silver staining using SilverQuest silver staining kit (Invitrogen, LC6070). ..

    Silver Staining:

    Article Title: MCM Double Hexamer Loading Visualised with Human Proteins
    Article Snippet: A sodium chloride wash was performed by washing 2x for 2 min, 1,250 rpm with bead-assay buffer + sodium chloride as indicated, followed by a LS wash. For elution, DNA was degraded by nuclease treatment by resuspending the beads in bead-assay buffer + 2 mM calcium chloride, 600 u micrococcal nuclease (NEB, M0247S) and incubation at 37°C for 2 min with 1,250 rpm shaking. .. The eluate was analysed by SDS-PAGE using 3-8% Criterion XT Tris-Acetate gels (Bio-Rad) and XT Tricine running buffer (Bio-Rad), followed by silver staining using SilverQuest silver staining kit (Invitrogen, LC6070). ..

    Membrane:

    Article Title: Variants in the proteasome regulator PSMF1 cause a phenotypic spectrum from parkinsonism to perinatal lethality.
    Article Snippet: RT-QuIC fluorescence responses were analyzed and plotted using GraphPad Prism 9.0 for Windows. .. Native gel analyses were performed to evaluate the relative abundance of different proteasome particles as previously described.19 5 to 10 μg of extracts from PSMF1 patient-derived fibroblasts and healthy controls dissolved in native sample buffer (Bio-Rad, 1610738) and separated on 3-8 % Criterion XT Tris-Acetate gels (Bio-Rad) under nondenaturing conditions and subsequently transferred onto PVDF membrane for standard Western blot analysis. ..

    Article Title: ATR, CHK1 and WEE1 inhibitors cause homologous recombination repair deficiency to induce synthetic lethality with PARP inhibitors
    Article Snippet: XT sample buffer (Bio-Rad Laboratories, Hercules, CA, United States) and XT reducing agent (Bio-Rad Laboratories, Hercules, CA, United States) were added at constants of 25% and 0.5%, respectively. .. Samples and HiMark pre-stained protein standard (Thermo Fisher Scientific, Waltham, MA, United States) were loaded onto 3−8% Criterion XT tris-acetate gels (Bio-Rad Laboratories, Hercules, CA, United States) which was run at 150 V for 1 h. Separated proteins on the gel were transferred onto nitrocellulose membrane (Amersham) at 100 V for 1 h before exposure to primary antibodies pCHK1S345 (Cell signalling #2348, diluted 1:1000), pCHK1S296 (Cell signalling #90178, diluted 1:1000), pCDK1Y15 (Cell signalling #9111, diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4 °C. .. Following washes, secondary anti-rabbit antibody (Dako #PO447, diluted 1:2000 in 5% milk powder in TBS-Tween) was added before further washes and the addition of Clarity Max ECL (Bio-rad, Hercules, CA, United States) to image membrane sections using the G Box (Syngene, Cambridge, UK).

    Article Title: ATR, CHK1 and WEE1 inhibitors cause homologous recombination repair deficiency to induce synthetic lethality with PARP inhibitors.
    Article Snippet: XT sample buffer (Bio-Rad Laboratories, Hercules, CA, United States) and XT reducing agent (Bio-Rad Laboratories, Hercules, CA, United States) were added at constants of 25% and 0.5%, respectively. .. Samples and HiMark pre-stained protein standard (Thermo Fisher Scientific, Waltham, MA, United States) were loaded onto 3−8% Criterion XT tris-acetate gels (Bio-Rad Laboratories, Hercules, CA, United States) which was run at 150 V for 1 h. Separated proteins on the gel were transferred onto nitrocellulose membrane (Amersham) at 100 V for 1 h before exposure to primary antibodies pCHK1S345 (Cell signalling #2348, diluted 1:1000), pCHK1S296 (Cell signalling #90178, diluted 1:1000), pCDK1Y15 (Cell signalling #9111, diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4 °C. .. Following washes, secondary anti-rabbit antibody (Dako #PO447, diluted 1:2000 in 5% milk powder in TBS-Tween) was added before further washes and the addition of Clarity Max ECL (Bio-rad, Hercules, CA, United States) to image membrane sections using the G Box (Syngene, Cambridge, UK).

    Western Blot:

    Article Title: Variants in the proteasome regulator PSMF1 cause a phenotypic spectrum from parkinsonism to perinatal lethality.
    Article Snippet: RT-QuIC fluorescence responses were analyzed and plotted using GraphPad Prism 9.0 for Windows. .. Native gel analyses were performed to evaluate the relative abundance of different proteasome particles as previously described.19 5 to 10 μg of extracts from PSMF1 patient-derived fibroblasts and healthy controls dissolved in native sample buffer (Bio-Rad, 1610738) and separated on 3-8 % Criterion XT Tris-Acetate gels (Bio-Rad) under nondenaturing conditions and subsequently transferred onto PVDF membrane for standard Western blot analysis. ..

    Modification:

    Article Title: BET Degraders Reveal BRD4 Disruption of 7SK and P-TEFb is Critical for Effective Reactivation of Latent HIV in CD4+ T-cells
    Article Snippet: .. Blots were performed as previously described ( ) with minor modification: proteins were separated by 4-20% TGX tris-glycine or 3-8% Criterion XT tris-acetate gels with tris/glycine/sds or tricine buffers respectively (BioRad) depending on size and membranes were developed using Supersignal West Atto or West Pico (ThermoFisher). .. Antibodies: BRD4L (Bethyl A700-005), BRD4L/S (Abcam ab128874), BRD2 (Abcam ab139690), BRD3 (ab50818), HEXIM1 (CST 12604), CDK9 (CST 2316S), CyclinT1 (CST 81464S), GAPDH (Abcam ab83956), aTubulin (Abcam ab7291), LaminB1 (Abcam ab133741), TBP (CST 44059S), Flag (Sigma F1804), V5 (Abcam ab27671).

    Article Title: BET degraders reveal BRD4 disruption of 7SK and P-TEFb is critical for effective reactivation of latent HIV in CD4+ T-cells.
    Article Snippet: .. Blots were performed as previously described (53) with minor modification: proteins were separated by 4%–20% TGX tris-glycine or 3%–8% Criterion XT tris-acetate gels with tris/glycine/sds or tricine buffers, respectively (BioRad), depending on size, and membranes were developed using Supersignal West Atto or West Pico (Ther moFisher). .. Antibodies: BRD4L (Bethyl A700-005), BRD4L/S (Abcam ab128874), BRD2 (Abcam ab139690), BRD3 (ab50818), HEXIM1 (CST 12604), CDK9 (CST 2316S), CyclinT1 (CST 81,464S), GAPDH (Abcam ab83956), aTubulin (Abcam ab7291), LaminB1 (Abcam ab133741), TBP (CST 44,059S), Flag (Sigma F1804), and V5 (Abcam ab27671).

    Article Title: BET degraders reveal BRD4 disruption of 7SK and P-TEFb is critical for effective reactivation of latent HIV in CD4+ T-cells
    Article Snippet: .. Blots were performed as previously described ( ) with minor modification: proteins were separated by 4%–20% TGX tris-glycine or 3%–8% Criterion XT tris-acetate gels with tris/glycine/sds or tricine buffers, respectively (BioRad), depending on size, and membranes were developed using Supersignal West Atto or West Pico (ThermoFisher). .. Antibodies: BRD4L (Bethyl A700-005), BRD4L/S (Abcam ab128874), BRD2 (Abcam ab139690), BRD3 (ab50818), HEXIM1 (CST 12604), CDK9 (CST 2316S), CyclinT1 (CST 81,464S), GAPDH (Abcam ab83956), aTubulin (Abcam ab7291), LaminB1 (Abcam ab133741), TBP (CST 44,059S), Flag (Sigma F1804), and V5 (Abcam ab27671).

    Control:

    Article Title: ATR, CHK1 and WEE1 inhibitors cause homologous recombination repair deficiency to induce synthetic lethality with PARP inhibitors
    Article Snippet: XT sample buffer (Bio-Rad Laboratories, Hercules, CA, United States) and XT reducing agent (Bio-Rad Laboratories, Hercules, CA, United States) were added at constants of 25% and 0.5%, respectively. .. Samples and HiMark pre-stained protein standard (Thermo Fisher Scientific, Waltham, MA, United States) were loaded onto 3−8% Criterion XT tris-acetate gels (Bio-Rad Laboratories, Hercules, CA, United States) which was run at 150 V for 1 h. Separated proteins on the gel were transferred onto nitrocellulose membrane (Amersham) at 100 V for 1 h before exposure to primary antibodies pCHK1S345 (Cell signalling #2348, diluted 1:1000), pCHK1S296 (Cell signalling #90178, diluted 1:1000), pCDK1Y15 (Cell signalling #9111, diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4 °C. .. Following washes, secondary anti-rabbit antibody (Dako #PO447, diluted 1:2000 in 5% milk powder in TBS-Tween) was added before further washes and the addition of Clarity Max ECL (Bio-rad, Hercules, CA, United States) to image membrane sections using the G Box (Syngene, Cambridge, UK).

    Article Title: ATR, CHK1 and WEE1 inhibitors cause homologous recombination repair deficiency to induce synthetic lethality with PARP inhibitors.
    Article Snippet: XT sample buffer (Bio-Rad Laboratories, Hercules, CA, United States) and XT reducing agent (Bio-Rad Laboratories, Hercules, CA, United States) were added at constants of 25% and 0.5%, respectively. .. Samples and HiMark pre-stained protein standard (Thermo Fisher Scientific, Waltham, MA, United States) were loaded onto 3−8% Criterion XT tris-acetate gels (Bio-Rad Laboratories, Hercules, CA, United States) which was run at 150 V for 1 h. Separated proteins on the gel were transferred onto nitrocellulose membrane (Amersham) at 100 V for 1 h before exposure to primary antibodies pCHK1S345 (Cell signalling #2348, diluted 1:1000), pCHK1S296 (Cell signalling #90178, diluted 1:1000), pCDK1Y15 (Cell signalling #9111, diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4 °C. .. Following washes, secondary anti-rabbit antibody (Dako #PO447, diluted 1:2000 in 5% milk powder in TBS-Tween) was added before further washes and the addition of Clarity Max ECL (Bio-rad, Hercules, CA, United States) to image membrane sections using the G Box (Syngene, Cambridge, UK).



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